Efficacy of epicardial implantation of acellular chitosan hydrogels in ischemic and nonischemic heart failure: impact of the acetylation degree of chitosan

This work explores the epicardial implantation of acellular chitosan hydrogels in two murine models cardiomyopathy, focusing on their potential to restore functional capacity heart. Different were generated using polymers four degrees acetylation, ranging from 2.5% 38%, because degree acetylation affects degradation and biological activity. The adjusted a 3% final polymer concentration. After complete macromolecular characterization chitosans study mechanical properties resulting hydrogels, they sutured onto surface myocardium, first rat after four-weeks coronary ligation (n=58) then mice with cardiomyopathy induced by cardiac-specific invalidation serum response factor (n=20). was associated reversion cardiac function loss maximal effects for 24%. extent fibrosis, cardiomyocyte length-to-width ratio, as well genes involved fibrosis stress repressed implantation. Our demonstrated beneficial particularly high remodeling models. findings indicate have great reliable therapeutic approach heart failure.